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KMID : 0364820150510020148
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Korean Journal of Microbiology
2015 Volume.51 No. 2 p.148 ~ p.155
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Molecular diversity of endobacterial communities in edible part of King oyster mushroom (Pleurotus eryngii) based on 16SrRNA
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Lee Choung-Kyu
Md. Azizul Haque
Choi Byoung-Rock
Lee Hee-Yul
Hwang Chung-Eun
Ahn Min-Ju
Cho Kye-man
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Abstract
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The diversity of endobacteria in the edible part (cap and stipe) king oyster mushroom (Pleurotus eryngii) was investigated using 16S rRNA sequence analysis. The bacterial 16S rRNA libraries were constructed from the body cap (BC) and the body stipe (BS) of the king oyster mushroom. The twenty sequenced BC clones were divided into four groups and the largest group was affiliated with the Firmicutes (40% of clones). While, the twenty sequenced BS clones could be divided into six groups and the largest group was affiliated with the Actinobacteria (40% of clones). The predominant bacterial family from both the cap and stipe of the mushroom was corresponded with the Gram positive bacteria (62.5%).
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KEYWORD
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Pleurotus eryngii, 16S rRNA gene, bacteria diversity, endobacteria, phylogenetic tree
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